Molecular epidemiology of Campylobacter jejuni isolates from the broiler production chain: first report of MLST profiles in Argentina

Campylobacter jejuni is an important foodborne pathogen with global distribution. We describe a genotyping study of collection C. (n = 137) isolated from different broiler farms and multiple sites along the processing line in slaughterhouse Argentina during 2011, 2012 2015. The isolates were genotyped using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST). Based on PFGE results, grouped into 26 pulsotypes. Subsequently, representing these pulsotypes chosen for MLST genotyping, which identified 16 types (STs) 6 clonal complexes (CCs) (21, 45, 48, 353, 354, 446). Several STs 7) have not been previously reported PubMLST.org database. most prevalent CCs 21, 45 (both associated human campylobacteriosis worldwide) 353. This showed high genetic diversity among production environment novel genotypes. es un importante agente patógeno de transmisión alimentaria nivel mundial. Describimos el estudio molecular una colección aislados diferentes granjas pollos y múltiples sitios frigorífico durante los años Los aislamientos fueron genotipados mediante electroforesis en campo pulsado tipificación secuencias Con base resultados PFGE, se agruparon pulsotipos. Se seleccionaron representativos dichos pulsotipos para genotipificarlos MLST, identificaron así secuenciotipos (ST) seis complejos clonales (CC) Varios ST no habían sido reportados previamente la datos PubMLST.org. CC más prevalentes (asociados con casos campilobacteriosis) Este identificó elevada diversidad genética cadena cárnica aviar argentina novedosos genotipos MLST.